The Lyra RSV + hMPV Assay is a multiplex real-time PCR assay for the qualitative detection and identification of respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) viral RNA extracted from nasal swabs, nasopharyngeal swabs, and nasal aspirate/wash specimens. This real time RSV + hMPV test detects RSV and hMPV and is able to differentiate between them.
A multiplex real-time RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for RSV, hMPV and the Process Control (PRC). Identification of RSV and hMPV and the PRC occurs by the use of target-specific primers and fluorescent-labeled probes that hybridize to conserved regions in the NS2 and polymerase genes of RSV and polymerase gene of hMPV and the PRC.
The Lyra RSV + hMPV Assay has a one-step reagent set up. Test results are available in less than 75 minutes after extraction. Lyra RSV + hMPV RT-PCR is available for use on the Applied Biosystems® 7500 Fast Dx, Biorad CFX 96, QuantStudioTM Dx Real-Time PCR Instrument and Cepheid® SmartCycler® II. The Lyra RSV + hMPV Assay is one in a growing menu of tests and intended to aid in the differential diagnosis of RSV and hMPV infections in humans.