The Sofia RSV Fluorescent Immunoassay (FIA) uses advanced immunofluorescence-based lateral-flow technology to detect respiratory syncytial virus (RSV) nucleoprotein antigen directly from nasopharyngeal swab and nasopharyngeal aspirate/wash specimens from symptomatic patients. The high sensitivity of the Sofia RSV FIA offers confidence in rapid RSV testing.
The Sofia RSV FIA involves the disruption of RSV viral antigens. To perform the rapid respiratory test, the patient specimen is placed in the Reagent Tube, during which time the virus particles in the specimen are disrupted, exposing internal viral nucleoproteins. After disruption, the specimen is dispensed into the Cassette sample well. From the sample well, the specimen migrates through a test strip containing various unique chemical environments. If RSV viral antigens are present, they will be trapped in a specific location.

Sofia will scan the test strip and measure the immunofluorescent signal by processing the results using method-specific algorithms and display the test results (Positive, Negative, or Invalid) on its display screen. The test results can also be automatically printed on an integrated printer if this option is selected.

RSV antigen
Fluorescent Immunoassay (FIA)
Nasopharyngeal swab, Nasopharyngeal aspirate/wash
Nasopharyngeal swab  86% / 97% ,
Nasopharyngeal wash/aspirate 89% / 98%
Quidel Corporation, USA

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